I-135

Material Data Sheet

Z-Leu-Leu-Nva-CHO (MG-115)

Price: $70.00
Catalog #: I-135

Z-Leu-Leu-Nva-CHO (MG-115)

Price: 
70
Quantity: 
5 mg
Data Sheet: 
application/pdf iconi-135-z-leu-leu-nva-cho-mg-115.pdf

Peptide aldehydes form a covalent hemiacetal adduct with the 20S proteasome, inhibiting the chymotrypsin-like peptidase activity. These inhibitors are reversible and cell-permeable, and also inhibit the activity of calpains and cathepsins.

Product Information

Structure: 
Purity: 
> 95% by HPLC.
Solubility: 
Soluble in DMSO up to 15 mg/ml. Pellet dry compound prior to reconstitution.
Activity: 

Ki = 20 nM vs. SDS-activated 20S proteasome.

Use & Storage

Use: 

Add from DMSO stock directly to in vitro or in vivo assays at desired concentration. Pre-incubation with compound prior to assay is recommended for maximal effect.

Storage: 
Store at -20°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Adams J., et al. (1998) Bioorg. Med. Chem. Lett. 8:333-338

Lee D.H. and Goldberg A.L. (1998) Tren. Cell. Biol. 8:397-403

Lee D.H. and Goldberg A.L. (1996) J. Biol. Chem. 271:27280-27284

McCormack T.A. et al, (1998) Biochem. 37:7792-7800

Wilk S., et al. (1993) Enz. Prot. 47:306-313