Material Data Sheet
Peptide aldehydes form a covalent hemiacetal adduct with the 20S proteasome, inhibiting the chymotrypsin-like peptidase activity. These inhibitors are reversible and cell-permeable, and also inhibit the activity of calpains and cathepsins.
Purity:> 95% by HPLC.
Solubility:Soluble in DMSO up to 15 mg/ml. Pellet dry compound prior to reconstitution.
Ki = 20 nM vs. SDS-activated 20S proteasome.
Use & Storage
Add from DMSO stock directly to in vitro or in vivo assays at desired concentration. Pre-incubation with compound prior to assay is recommended for maximal effect.
Store at -20°C. Avoid multiple freeze/thaw cycles.
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