I-130

Material Data Sheet

Z-Leu-Leu-Leu-CHO (MG-132)

Price: $30.00
Catalog #: I-130

Z-Leu-Leu-Leu-CHO (MG-132)

Price: 
$30 $70
Quantity: 
1 mg 5mg
Data Sheet: 
application/pdf icon130_MG132.pdf
Structure: 
Purity: 
> 95% by HPLC.
Solubility: 
Soluble in DMSO up to 15 mg/ml. Note: Pellet may have oily appearance.
Activity: 
Ki = 4.2 nM vs. SDS-activated 20S proteasome. IC50 in cell culture = 5 μM.

Peptide aldehydes form a covalent hemiacetal adduct with the 20S proteasome, inhibiting the chymotrypsin-like peptidase activity. These inhibitors are reversible and cell-permeable, and also inhibit the activity of calpains and cathepsins.

Use & Storage

Use: 
Add from DMSO stock directly to in vitro or in vivo assays at desired
concentration. Pre-incubation with compound prior to assay is recommended
for maximal effect.
Storage: 
Store at -20°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Adams J., et al. (1998) Bioorg. Med. Chem. Lett. 8:333-338

Lee D.H. and Goldberg A.L. (1998) Tren. Cell. Biol. 8:397-403

Lee D.H. and Goldberg A.L. (1996) J. Biol. Chem. 271:27280-27284

McCormack T.A. et al, (1998) Biochem. 37:7792-7800

Wilk S., et al. (1993) Enz. Prot. 47:306-313