I-130

Material Data Sheet

z-Leu-Leu-Leu-CHO (MG-132)

Price: $125.00
Catalog #: I-130

z-Leu-Leu-Leu-CHO (MG-132)

Price: 
$125
Quantity: 
5 mg
Data Sheet: 

MG-132 is a peptide aldehyde that forms a covalent hemiacetal adduct with the 20S proteasome, inhibiting the chymotrypsin-like peptidase activity (IC50 = 100 nM).  MG-132 inhibition is reversible and the compound is cell-permeable.  MG-132 also inhibits calpain activity (IC50 = 1 µM) and cathepsin activity.  This compound inhibits TNFα-induced NFκB activation and IκB degradation, and induces neurite outgrowth in PC12 cells.

Product Information

MW: 
475.6 Da
Purity: 
>95% by HPLC
Solubility: 
Soluble in Ethanol and DMSO to at least 100 mM
Activity: 

Ki = 4.2 nM vs SDS-activated 20S proteasome

Use & Storage

Use: 

Add from reconstituted stock directly to in vitro or cell-based assays.  

Storage: 

Store at -20°C.  Avoid multiple freeze-thaw cycles.

Literature

References: 

Adams J. et al.  (1998) Bioorg. Med. Chem. Lett.  8: 333

Lee D.H. & Goldberg A.L. (1998) Trends Cell Biol.  8: 397

Lee D.H. & Goldberg A.L. (1998) J. Biol. Chem.  271: 27280

McCormack T.A. et al. (1998) Biochem.  37: 7792

 

Wilk S. et al. (1993) Enz. Prot.  47: 306