F-375

Material Data Sheet

Protein Fraction I (Ub, E2, E3)

Price: $100.00

Catalog #: F-375

Protein Fraction I (Ub, E2, E3)

Price:

$100 $200

Quantity:

1 mg 2.5 mg

Data Sheet:

375_hFI.pdf

Stock:

X mg/ml in 50 mM HEPES pH 8.0.
Actual protein concentration will vary with specific lot number.

Also known as: FI

Protein fraction of a cell extract that does not bind to anion exchange resin. Contains ubiquitin and fraction I E2 and E3s enzymes. FI is essentially free of ATP.

Use & Storage

Use:

Ideal for demonstrating if FI enzymes are critical for degradation of in vitro
substrates as well as in vitro conjugation activity. Isopeptidase inhibitor (U-201) and a proteasome inhibitor are highly recommended for the efficient accumulation of ubiquitin-protein conjugates. May require FII (F-370) for activity in the presence of proteasome and isopeptidase inhibitors. A typical assay concentration for FI is 0.5-4 mg/ml. [Note: FI may have a cloudy appearance. This is normal.]

Storage:

Store at -80°C. For best results, aliquot and freeze after first set of experiments to avoid multiple freeze/thaw cycles.

Literature

References:

Hersko A., et al. (1982) J. Biol. Chem. 258:8206-8214