Material Data Sheet

S-100 Fraction

Price: $149.00
Catalog #: F-372

S-100 Fraction

5 mg
Data Sheet: 

Cell fraction containing the full HeLa cell compliment of E1, E2s, E3s, 20S and 26S proteasomes, isopeptidases, and PA28. Useful in assays to demonstrate ubiquitin conjugation and degradation.

Product Information

X mg/ml in 50 mM HEPES pH 7.6, 20mM NaCl, 0.5 mM DTT.Actual concentration varies with lot number. 

Use & Storage


Ideal for demonstrating ubiquitin proteasome degradation/ conjugation of radiolabeled or immuno-detectable substrates. Typical assay concentration for extract is 0.5-4 mg/ml. Isopeptidase inhibitor (U-201) and a proteasome inhibitor are highly recommended for the efficient accumulation of ubiquitinprotein conjugates.

Store at -80°C. For best results, aliquot and freeze after first set of experiments to avoid multiple freeze/thaw cycles.



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