F-372

Material Data Sheet

S-100 Fraction

Price: $165.00
Catalog #: F-372

S-100 Fraction

Price: 
$165
Quantity: 
2.5 mg
Data Sheet: 
application/pdf icon372_s100FII.pdf
Stock: 
X mg/ml in 50 mM HEPES pH 7.6, 20mM NaCl, 0.5 mM DTT.
Actual concentration varies with lot number. 

Cell fraction containing the full HeLa cell compliment of E1, E2s, E3s, 20S and 26S proteasomes, isopeptidases, and PA28. Useful in assays to demonstrate ubiquitin conjugation and degradation.

Use & Storage

Use: 
Ideal for demonstrating ubiquitin proteasome degradation/ conjugation of radiolabeled or immuno-detectable substrates. Typical assay concentration for extract is 0.5-4 mg/ml. Isopeptidase inhibitor (U-201) and a proteasome inhibitor are highly recommended for the efficient accumulation of ubiquitinprotein conjugates.
Storage: 
Store at -80°C. For best results, aliquot and freeze after first set of experiments to avoid multiple freeze/thaw cycles.

Literature

References: 

Hersko A., et al. (1982) J. Biol. Chem. 258:8206-8214
Driscoll J. and Goldberg A.L. (1990) J. Biol. Chem. 265:4789-4792
Ganoth D., et al. (1988) J. Biol. Chem. 263:12412-12419
Wing S.S. (2003) Intl. J. Biochem. Cell. Biol. 35:590-605
Voges D., et al. (1999) Ann. Rev. Biochem. 68:1015-1068
Waxman L., et al. (1987) J. Biol. Chem. 262:2451-2457
Weissman A.M. (2001) Nat. Rev. 2:169-178