F-370

Material Data Sheet

Protein Fraction II

Price: $100.00
Catalog #: F-370

Protein Fraction II

Price: 
$100 $200
Quantity: 
1 mg 2.5 mg
Data Sheet: 
application/pdf icon370_hFII.pdf
Stock: 
10 mg/ml in 25 mM HEPES pH 7.6
Actual protein concentration will vary with specific lot number. 

Also known as: FII

Protein fraction from a cell extract that binds to an anion exchange resin. Essentially ubiquitin-free and ATP-free, FII contains E1, most E2s, some E3s, UCHs, and the proteasome (20S and 26S).

Use & Storage

Use: 
Ideal for demonstrating ubiquitin- and ATP-dependent degradation/conjugation of radio-labeled or immuno-detectable substrates. Isopeptidase inhibitor (U- 201) and a proteasome inhibitor are highly recommended for the efficient accumulation of ubiquitin-protein conjugates. A typical assay concentration for FII is 0.5-4 mg/ml.
Storage: 
Store at -80°C. For best results, aliquot and freeze after first set of experiments to avoid multiple freeze/thaw cycles.

Literature

References: 

Hersko A., et al. (1982) J. Biol. Chem. 258:8206-8214