F-365

Material Data Sheet

26S Proteasome Fraction

Price: $275.00

Catalog #: F-365

26S Proteasome Fraction

Price:

$275

Quantity:

200 μL

Data Sheet:

365_26S.pdf

Stock:

Estimated 26S concentration = 100 nM. Concentration will vary with lot number.
Buffer: 50 mM Hepes pH 7.6, 1 mM MgATP, 1 mM DTT,
150 mM NaCl, 10 % glycerol.

Mammalian 26S proteasome purified through a specific series of specialized chromatographic steps yields a partially purified fraction containing 26S proteasome and no conjugation enzymes (E1, E2s, and E3s). This stable fraction is preferred for the degradation of pre-formed ubiquitin protein conjugates by the 26S proteasome since highly purified 26S is costly and more labile. 26S concentration is estimated based on analytical SEC at 1500 kDa and quantitation of the 20S core particle activity using a fluorogenic peptide substrate.

Use & Storage

Use:

For best results, aliquot and freeze after first set of experiments to avoid multiple freeze/thaw cycles. Typical concentrations for use are 5-50 nM depending on conditions.

Storage:

Store at -80°C. Once thawed, keep on ice prior to use.

Literature

References:

Driscoll J. and Goldberg A.L. (1990) J. Biol. Chem. 265:4789-4792
Ganoth D., et al. (1988) J. Biol. Chem. 263:12412-12419
Voges D., et al. (1999) Ann. Rev. Biochem. 68:1015-1068
Waxman L., et al. (1987) J. Biol. Chem. 262:2451-2457