F-360

Material Data Sheet

Protein Fraction II (E1, E2, E3, UCHs, 20S/26S)

Price: $165.00

Catalog #: F-360

Protein Fraction II (E1, E2, E3, UCHs, 20S/26S)

Price:

$165 $270

Quantity:

2.5 mg 5 mg

Data Sheet:

360_FII.pdf

Stock:

10.0 mg/ml in 25 mM HEPES pH 7.6.
Actual protein concentration will vary with specific lot number.

Also known as: FII

Protein fraction from a cell extract that binds to an anion exchange resin. Essentially ubiquitin-free and ATP-free, FII contains E1, most E2s, some E3s, UCHs, and the proteasome (20S and 26S).

Use & Storage

Use:

Ideal for demonstrating ubiquitin- and ATP-dependent degradation/conjugation of radio-labeled or immuno-detectable substrates. The inclusion of an isopeptidase inhibitor and a proteasome inhibitor are highly recommended for the efficient accumulation of ubiquitin protein conjugates. Typical assay concentrations for FII are 0.5 - 4 mg/ml.

Storage:

Store at -80°C. For best results, aliquot and freeze after first set of experiments to avoid multiple freeze/thaw cycles.

Literature

References:

Hersko A., et al. (1982) J. Biol. Chem. 258:8206-8214