E3-655

New
Material Data Sheet

VHL/ELOB/ELOC/CUL2/RBX1 Complex

Price: $0.00
Catalog #: E3-655

VHL/ELOB/ELOC/CUL2/RBX1 Complex

Price: 
$285
Quantity: 
25 µg
Data Sheet: 

VHL (von Hippel-Lindau disease tumor suppressor) is the substrate recognition subunit for an E3 ligase activity that ubiquitinates proteins containing hydroxyproline residues.  Targets of VHL include HIF1α, β2 adrenergic receptor, ZHX2 and others.  In VHL-box E3 Ubiquitin ligases, VHL is bound to a heterodimer complex composed of Elongin B (ELOB) and Elongin C (ELOC) through the “BC-box motif” found in many proteins in the VHL-box and SOCS-box protein families.  ELOB/ELOC serves as an adapter between substrate recognition proteins and Cullin-2 (or Cullin-5) and RBX1 to form an active E3 ligase.  This protein complex contains an amino-terminal His-tag on the CUL2 subunit.

Product Information

Stock: 
X mg/ml (X µM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 2 mM DTT
MW: 
24 kDa (VHL), 13.1 kDa (ELOB), 12.8 kDa (ELOC), 93 kDa (CUL2), 12 kDa (RBX1)
Purity: 
> 90% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain
Storage: 

Store at -80°C and avoid multiple freeze/thaw cycles