E3-650

New
Material Data Sheet

CUL4A/RBX1/DDB1/CRBN

Price: $285.00
Catalog #: E3-650

CUL4A/RBX1/DDB1/CRBN

Price: 
$285
Quantity: 
25 µg
Data Sheet: 

Cullin-4A (CUL4A) is a core component of multiple cullin-RING type Ubiquitin ligase complexes that mediate the ubiquitination of proteins involved in cell cycle progression, DNA repair and other processes.  In the DCX complex (DDB1-CUL4-X-box), CUL4A serves as a scaffold that organizes the DDB1-X-box substrate recognition subunits with the RBX1 subunit and contributes to catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme.  In vivo, the E3 ubiquitin ligase activity of the DCX complex is dependent on neddylation of the CUL4 subunit, though neddylation may be dispensable in some in vitro reactions.  This complex consists of an N-terminal 10-His tagged, Cullin-4A (UniProt Q13619), untagged RBX1 (UniProt P62877), untagged DDB1 (UniProt Q16531) and untagged CRBN (UniProt Q96SW2)

Product Information

Stock: 
X mg/ml (X µM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
MW: 
283 kDa
Purity: 
> 85% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro will depend on experimental conditions.

Storage: 

Store at -80°C and avoid multiple freeze/thaw cycles.