E3-600

New
Material Data Sheet

Elongin B/C-VHL Complex

Price: $285.00
Catalog #: E3-600

Elongin B/C-VHL Complex

Price: 
$285
Quantity: 
50 µg
Data Sheet: 

Together, Elongin B (ELOB) and Elongin C (ELOC) form a heterodimer that serves as the regulatory subunit for the elongin complex—a general transcription elongation factor that increases RNA Polymerase II transcription through template-encoded arresting sites.  The ELOB/ELOC complex also binds to the “BC-box motif” found in many proteins in the VHL-box and SOCS-box protein families.  In this function, ELOB/ELOC serves as an adapter between substrate recognition proteins and either Cullin-2/Rbx1 (in VHL-box E3 Ubiquitin ligases) or Cullin-5/Rbx2 (in SOCS-box E3 Ubiquitin ligases).  VHL (von Hippel-Lindau disease tumor suppressor) is the substrate recognition subunit for an E3 ligase activity that ubiquitinates proteins containing hydroxyproline residues.  Targets of VHL include HIF1α, β2 adrenergic receptor, ZHX2 and others.  This protein complex is untagged.

Product Information

Stock: 
X mg/ml (X µM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 2 mM DTT
MW: 
24 kDa (VHL), 13.1 kDa (ELOB), 12.8 kDa (ELOC)
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain
Storage: 

Store at -80°C and avoid freeze/thaw cycles