E3-420

New
Material Data Sheet

CUL2/RBX1 Complex

Price: $285.00
Catalog #: E3-420

CUL2/RBX1 Complex

Price: 
$285
Quantity: 
25 µg
Data Sheet: 

Cullin-2 (CUL2) is a core component of multiple cullin-RING based “ECS” (Elongin B/C-CUL2-SOCS-box protein) E3 Ubiquitin ligase complexes that mediate the ubiquitination of proteins involved in a variety of cellular processes. In the ECS complex, Cullin-2 serves as a scaffold that organizes the ELOB/C-associated recognition subunits with the RBX1 subunit and contributes to catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme.  In vivo, the E3 ubiquitin ligase activity of the ECS complex is dependent on neddylation of the cullin subunit, though neddylation may be dispensable for some in vitro reactions.  This complex consists of an N-terminal 10-His tagged Cullin-2 (UniProt Q13617) and untagged RBX1 (UniProt P62877).

Product Information

Stock: 
X mg/ml (X µM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
MW: 
93 kDa (CUL2), 12 kDa (RBX1)
Purity: 
> 90% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro will depend on experimental conditions.

Storage: 

Store at -80°C and avoid freeze/thaw cycles.