E3-411

New
Material Data Sheet

CUL1 (Neddylated)/RBX1 Complex

Price: $449.00
Catalog #: E3-411

CUL1 (Neddylated)/RBX1 Complex

Price: 
$449
Quantity: 
25 µg
Data Sheet: 

Cullin-1 (CUL1) is a core component of multiple SCF (SKP1-CUL1-F-box) E3 Ubiquitin ligase complexes that mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.  In the SCF complex, CUL1 serves as a scaffold that organizes the SKP1-F-box recognition subunits with the RBX1 subunit and contributes to catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme.  In vivo, the E3 ubiquitin ligase activity of the SCF complex is dependent on neddylation of the cullin subunit, though neddylation may be dispensable for some in vitro reactions.  This complex consists of an N-terminal 10-His tagged CUL1 (UniProt Q13616) and untagged RBX1 (UniProt P62877).  CUL1 is neddylated (Q15843) in this complex.

Product Information

Stock: 
X mg/ml (X µM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
MW: 
105 kDa (Neddylated-CUL1), 12 kDa (RBX1)
Purity: 
>90% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue Stain

Use & Storage

Use: 

Store at -80°C