E3-302

Material Data Sheet

HECTD2

Price: $185.00
Catalog #: E3-302

HECTD2

Price: 
$185
Quantity: 
25 µg
Data Sheet: 

HECTD2 (aka "HECT domain-containing protein 2" or "HECT-type E3 ubiquitin transferase HECTD2") is a 776 amino acid member of the HECT (Homologous to E6AP Carboxyl Terminus) domain family of Ubiquitin ligases.  With a predicted weight of 88 kDa, human HECTD2 shares 89% identity and 94% similarity with the mouse orthologue.  In innate immunity HECTD2 exerts a pro-inflammatory effect by contributing to the ubiquitylation and degradation of PIAS1, a multifunctional and potent anti-inflammatory protein that negatively regulates several key inflammatory pathways.  Small molecule inhibitors of HECTD2 were found to attenuate lipopolysaccharide (LPS)-induced lung inflammation.  HECTD2 has also been shown to ubiquitylate BoNT/A, a catalytic light chain of botulinum neurotoxin.  Ubiquitylation can lead to the proteasomal degradation of the light chain, but it's rescued from destruction by the dominant effect of a deubiquitinating enzyme, VCIP135/VCPIP1.

Product Information

Stock: 
X mg/ml (X µM) in 50 mM HEPES pH 7.0, 100 mM NaCl, 5% (v/v) Glycerol,  5 mM DTT
MW: 
88 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Typical enzyme concentration for in vitro conjugation reactions is 100-500 nM depending on experimental conditions and substrate.

Storage: 

Store at -80ºC.  Avoid freeze/thaw cycles