E3-300

New
Material Data Sheet

Livin β/BIRC7 isoform 1

Price: $185.00
Catalog #: E3-300

Livin β/BIRC7 isoform 1

Price: 
$185
Quantity: 
50 µg
Data Sheet: 
application/pdf icone3-300.pdf

Livin α and Livin β are two splice variants generated from the Baculoviral IAP repeat-containing protein 7 (BIRC7) gene.  Livin is a member of the Inhibitor of Apoptosis Protein (IAP) family that contains a single baculovirus IAP repeat (BIR) and a C-terminal RING domain.  Livin exerts an anti-apoptotic activity by inhibiting caspase's 3, 7, and 9, and by its E3 Ubiquitin ligase activity.  In vitro, Livin directly inhibits caspase 3 and proteolytic activation of pro-caspase 9, and ubiquitinates recombinant DIABLO.  Livin ß is a splice variant that's 18 amino acids shorter in the BIR-RING linking region than the Livin α splice variant.  This recombinant protein contains a C-terminal 6-His tag.

Product Information

Stock: 
X mg/ml (X µM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM DTT
MW: 
32 kDa
Purity: 
> 90% by SDS-PAGE gel under reducing conditions and visualized by Colloidal Coomassie Blue Stain

Use & Storage

Use: 

Recombinant Human Livin β is a Ubiquitin Ligase (E3) that functions downstream of a Ubiquitin-activating (E1) enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins.  Reaction conditions will need to be optimized for each specific application.

Storage: 

Store at -80ºC and avoid multiple freeze/thaw cycles.