E3-280

New
Material Data Sheet

cIAP-1/HIAP-2

Price: $0.00
Catalog #: E3-280

cIAP-1/HIAP-2

Price: 
$179
Quantity: 
50 µg
Data Sheet: 

Cellular inhibitor of apoptosis protein 1 (cIAP-1, also known as BIRC2, MIHB, and HIAP2) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. Structurally, cIAP-1 is comprised of 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD).  Functionally, cIAP-1 inhibit caspases through the direct interaction of its BIR domain with the active caspase.  The ring finger domain of cIAP-1 also functions as an E3 ubiquitin ligase to ubiquitinate specific target proteins. Caspase activity may be restored by mitochondrial proteins, such as SMAC/Diablo or HtrA2/Omi, through interactions with the Reaper-like motif and the BIR domain.

Product Information

Stock: 
X mg/ml (X µM) in 50mM HEPES pH 8.0, 500 mM NaCl, 10% (v/v) Glycerol, 1mM TCEP 
MW: 
72 kDa
Purity: 
> 85% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Recombinant Human cIAP-1/HIAP-2 is a RING finger Ubiquitin ligase (E3) that functions downstream of a Ubiquitin-­activating (E1) enzyme and a Ubiquitin-­conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial cIAP-1 concentration of 0.2-­1 µM.  

Storage: 

Store at -80°C.  Avoid multiple freeze-thaw cycles.