E3-270

Material Data Sheet

UBE4B, His6-tag

Price: $275.00
Catalog #: E3-270

UBE4B, His6-tag

Price: 
$275
Quantity: 
50 µg
Data Sheet: 

Ubiquitin conjugation factor E4 B (UBE4B, also known as Ubiquitin fusion degradation protein 2) is a recently identified E3 and E4 ubiquitin ligase that physically interacts with p53 and Mdm2 to promote p53 polyubiquitination and degradation. Recent work in mouse models suggests that p53 loss in accelerated medulloblastomas is driven by UBE4B.  Overexpression of UBE4B correlated with decreased expression of p53 in xenotransplant tumor models, and overexpression of UBE4B is often observed in human brain tumors. This protein seems to be involved in multiple pathways that are all associated with neuronal survival and degradation.

Product Information

Stock: 
X mg/ml (X µM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 20% (v/v) Glycerol, 1 mM TCEP, 10 μM ZnCl2
MW: 
147 kDa
Purity: 
> 90% by SDS-PAGE gel under reducing conditions and visualized by Colloidal Coomassie Blue Stain

Use & Storage

Use: 

Typical enzyme concentration for in vitro conjugation reactions is 100-500 nM depending on experimental conditions and substrate.  Recombinant human UBE4B demonstrates strong in vitro autoubiquitination activity in the presence of UBE1, UBE2D3, Ubiquitin, and ATP.

Storage: 

Store at -80ºC.  Avoid freeze/thaw cycles