E3-240

Material Data Sheet

His6-MBP-HOIP Catalytic Domain

Price: $269.00
Catalog #: E3-240

His6-MBP-HOIP Catalytic Domain

Price: 
$269
Quantity: 
100 µg
Data Sheet: 

HOIP (HOIL-1 Interacting Protein, also known as RNF31) is a RING domain ubiquitin E3 ligase and component of the LUBAC complex.  LUBAC, a large (approximately 600 kDa) heteromultimeric assembly consisting of at least HOIP, HOIL-1, and Sharpin, conjugates linear polyubiquitin chains to various substrates and plays a key role in the NF-κB activation and signaling events mediated through TNFα and IFN.  Both the catalytic activity and specificity for linear ubiquitin chain formation of LUBAC are contained within the C-terminal RBR (RING-IBR-RING) and LDD (linear ubiquitin chain determining domain) domains of HOIP.  HOIL-1 and Sharpin are essential for full-length HOIP activity, but not for HOIP truncation mutants consisting of the RBR and LDD domains.  This protein contains N-terminal 6-His and MBP tags, and is useful for generating unachored linear polyubiquitin chains without the requirement for HOIL-1 or Sharpin.  HOIP catalytic domain contains aa's 699-1072 (Accession Number Q96EP0)

Product Information

Stock: 
X mg/ml (X mM) in 50 mM Hepes pH 7.5, 150 mM NaCl, 10% glycerol, 1 mM TCEP
MW: 
87 kDa
Purity: 
>90% by SDS-PAGE

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 0.1-0.5 μM depending on experimental conditions.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Belgnaoui S.M., et al.  (2012) Cell Host Microbe   12: 211-222

Smit J.J, et al.  (2012)  EMBO J.  31: 3833–3844

Stieglitz B., et al.  (2012)   EMBO Rep.  13: 840-846

Wauer T. and Komander D.  (2013) EMBO J.  doi:10.1038/emboj.2013.125