E3-230

Material Data Sheet

E6AP/UBE3A

Price: $279.00
Catalog #: E3-230

E6AP/UBE3A

Price: 
$279
Quantity: 
50 μg
Data Sheet: 

E6AP (E6-Associated Protein) is a HECT domain ubiquitin E3 ligase that ubiquitinates and mediates the proteasomal destruction of substrate proteins. HECT domain ligases use an active site cysteine to accept charged ubiquitin from ubiquitin-E2 thioester complexes for subsequent transfer to substrate proteins; in this way HECT class ligases are distinct from most RING class ligases in that the latter facilitate transfer of ubiquitin from charged E2’s directly to substrate proteins without an E3-ubiquitin thioester intermediate. The viral HPV-E6 protein (present in cells infected with human papillomavirus) forms a complex with E6AP to generate a ligase activity that polyubiquitinates the tumor suppressors p53 and p73 and targets them to the 26S proteasome for degradation. This protein contains a C-terminal 6-His tag.

Product Information

Stock: 
X mg/ml (X mM) in 50 mM Hepes pH 8.0, 100 mM NaCl, 1 mM TCEP
MW: 
102 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 1-5 μM depending on experimental conditions.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Huang L., et al.  (1999)  Science   286: 1321-1326

Martinez-Noel G., et al.   (2012)   Mol. Cell. Biol.   32: 3095-3106

Mishra A., et al.  (2009)   J Biol Chem.  284: 10537-10545