E3-220

Material Data Sheet

CHIP/Stub1

Price: $269.00
Catalog #: E3-220

CHIP/Stub1

Price: 
$269
Quantity: 
50μg
Data Sheet: 

CHIP (Carboxy terminus of Hsp70-Interacting Protein) is a U-Box ubiquitin E3 ligase that ubiquitinates and mediates the proteasomal destruction of misfolded chaperone substrates. CHIP functions in coordination with several chaperone complexes, including Hsp40, Hsp70, and Hsp90. CHIP activity may be modulated by the deubuiquitinase Ataxin-3, which restricts the length of ubiquitin chains attached to CHIP substrates and prevents further chain extension. This protein contains a C-terminal 6-His tag.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM Hepes pH 8.0, 100 mM NaCl, 5 mM DTT, 10% glycerol
MW: 
36 kDa
Purity: 
> 90% by SDS-PAGE

Use & Storage

Use: 

 

Typical enzyme concentration to support conjugation in vitro is 0.1-5 μM depending on experimental conditions.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Connell P., et al. (2001) Nat. Cell Bio. 3: 93-96

Qian S.B., et al. (2006) Nature 440: 551-555

Scaglione K.M., et al. (2011) Mol. Cell 43: 599-612