E3-210

Material Data Sheet

RNF4

Price: $269.00
Catalog #: E3-210

RNF4

Price: 
$269
Quantity: 
50 µg
Data Sheet: 

RNF4 (small nuclear ring finger protein, SNURF) is a RING-finger ubiquitin E3 ligase that ubiquitinates and mediates the proteasomal destruction of targets such as PML, PEA3, CENP1, and PARP1.  In addition to the RING domain, RNF4 contains four SUMO-interacting motifs (SIMs) that function to recruit this ligase to poly-sumoylated substrates.  RNF4 will autoubiquitinate in vitro, and will also ubiquitinate poly-SUMO chains.  This recombinant protein is untagged. 

Product Information

Stock: 
X mg/ml (X µM) in 50 mM Hepes 8.0, 200 mM NaCl, 5 mM DTT, 50 µM ZnCl2
MW: 
21 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 50-500 nM depending on experimental conditions.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Geoffroy, M-C., et al. (2010) Mol. Bio. Cell 21: 4227-4239

Tathum, M.H., et al. (2008) Nat. Cell Bio. 10: 538-546