E3-202

Material Data Sheet

GST-Mdm2/HDM2

Price: $279.00
Catalog #: E3-202

GST-Mdm2/HDM2

Price: 
$279
Quantity: 
50 µg
Data Sheet: 

Double minute 2 protein (Mdm2, also known as HDM2) is a RING-finger Ubiquitin E3 ligase that acts as a major regulator of the tumor suppressor p53.  Mdm2 inhibits p53-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain.  The E3 ligase activity is confined to the C-terminal domain of Mdm2 and is responsible for the ubiquitination and subsequent proteasomal degradation of p53.  Although the isolated RING domain is capable of p53 ubiquitination, other regions of the protein including a central acidic domain are also crucial for full E3 ligase activity.  Mdm2 also regulates its own intracellular levels by auto-ubiquitination, and can be SUMOylated which decreases autoubiquitinylation activity but increases activity toward p53.  Mdm2 also affects the cell cycle and apoptosis through interactions with other proteins such as retinoblastoma1 (pRB) and ribosomal protein L5.  This recombinant protein contains an N-terminal GST tag.

Product Information

Stock: 
X mg/ml (X mM) in 50 mM Hepes pH 8, 200 mM NaCl, 10% glycerol, 5 mM TCEP
MW: 
83 kDa
Purity: 
>85% by SDS-PAGE

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 0.5-5 mM depending on conditions.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Brooks C.L., et al. (2007) J.Biol.Chem  282: 22804-22815

Bushmann T., et al. (2001) J.Biol.Chem  276: 40389-40394

Dornan D, et al. (2004) Nature 429:86-92

Grossman S.R., et al. (2003) Science 300:342-344

Hu M., et al (2006)  PLoS Biol. 4:228-239

Kawai H., et al. (2003) Mol. Cell. Biol. 23: 4939-4947

Lai Z., et al. (2001) J.Biol.Chem  276: 31357-31367

Li M, et al. (2003) Science 301:1972-1975

Linares L.K., et al. (2003) Proc.Natl.Acad.Sci.  100:12009-12014

Sheng Y, et al. (2006) Nat.Struc.Mol.Biol. 13:285-291

Wu H., et al. (2011) Nature Med.  17:  347-356

Yan Y. and Chen J. (2005) Biochem. Biophys. Res. Comm.  332:702-709

Yu G.W., et al.  (2006) Proc.Natl.Acad.Sci. 103:1227-1323