E2-750

Material Data Sheet

His6-UBE2J1

Price: $229.00
Catalog #: E2-750

His6-UBE2J1

Price: 
$229
Quantity: 
100 µg
Data Sheet: 

UBE2J1 is a human homologue of the yeast Ubc6 protein.  In yeast, this tail-anchored transmembrane E2 is localized to the cytoplasmic surface of the ER and participates in ER-associated degradation (ERAD) of misfolded proteins.  UBE2J1 is reported to play a role in degradation of misfolded MHC class I molecules via a mechanism requiring the E3 ubiquitin ligase Hrd-1.  The C-terminal transmembrane and lumenal amino acid sequences have been removed from this recombinant protein.

Product Information

Stock: 
X mg/ml (X µM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 2 mM DTT
MW: 
32 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 µM depending on conditions.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.