E2-740

Material Data Sheet

His6-UBE2W (UBC16)-1

Price: $229.00
Catalog #: E2-740

His6-UBE2W (UBC16)-1

Price: 
$229
Quantity: 
100ug

UBE2W (UBC16) may play a role in Fanconi anemia tumor suppressor pathway by mono-ubiquitinating FANCD2 when in complex with the E3 ligase FANCL.  This E2 has also been shown to catalyze the autoubiquitination of the BRCA1/BARD1 E3 complex, and to form K11 linked poly-Ubiquitin chains in vitro.

Product Information

Stock: 
X mg/ml (X µM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 2 mM DTT
MW: 
18 kDa
Purity: 
> 90% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 µM depending on conditions.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.