E2-695

Material Data Sheet

His6-UBE2T

Price: $100.00
Catalog #: E2-695

His6-UBE2T

Price: 
$100 $200
Quantity: 
50 μg 100 μg
Data Sheet: 
application/pdf icon695_Ube2T.pdf
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 6.8, 50 mM NaCl, 10% glycerol, 1 mM DTT. Concentration varies with lot number.

MW: 
24.5 kD
Purity: 
> 95% by SDS-PAGE

Ube2T (HSPC150) was cloned from CD34-positive hematopoietic stem cells. Ube2T homologs are only found in vertebrates and have widespread expression. Ube2T is the E2 enzyme which is essential for the Fanconi anemia pathway, required for the efficient repair of damaged DNA. Ube2T binds to FANCL, the ubiquitin ligase subunit of the Fanconi anemia core complex, and is required for the mono-ubiquitination of FANCD2 in vivo. In addition, Ube2T can mono-ubiquitination itself in vivo which inactivates the E2. Ube2T. This self-inactivation mechanism could be important for negative regulation of the Fanconi anemia pathway. This protein has an N-terminal His6-tag. Accession # NP_006348.1.

Use & Storage

Use: 
Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.
Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Alpi A.F., et al. (2007) Mol. Cell Biol. 27:8421-8430
Alpi A.F., et al. (2008) Mol. Cell 32:767-777
Hao J., et al. (2008) Tumour Biol. 29:195-203
Longerich S., et al. (2009) J. Biol. Chem.
Machida Y.J., et al. (2006) Mol. Cell 23:589-596
Zhang J., et al. (2007) Cell. Cycle 7:407-413
Zhang J., et al. (2007) J. Genet. Genomics. 34:573-580