E2-690

Material Data Sheet

His6-UBE2S

Price: $229.00
Catalog #: E2-690

His6-UBE2S

Price: 
$229
Quantity: 
100 µg
Data Sheet: 

UBE2S (E2-24K, E2-EPF) shares 38% identity with S.cerevisiae UBC4 with a conserved UBC domain that is characteristic of Ubiquitin E2 conjugating enzymes. This E2 enzyme is over-expressed in common human cancers and targets von Hippel-Lindau (VHL) for proteosomal degradation, thereby stabilizing HIF-1α.  Overexpression of UBE2S in vitro and in vivo boosts tumor-cell proliferation, invasion and metastasis through effects on the pVHL-HIF pathway, and may thus be a novel molecular target for therapeutic intervention. UBE2S has also been identified as a K11-specific E2 for the anaphase-promoting complex (APC/C), and the E2 depends on the cell cycle-dependent association with the APC/C activators Cdc20 and Cdh1 for its activity.  In vitro, UBE2S elongates ubiquitin chains initiated by the E2 enzymes UbcH10 and UbcH5, enhancing the degradation of APC/C substrates by the proteasome.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 50 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
MW: 
26 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions. 

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.