E2-680

Material Data Sheet

His6-UBE2G2

Price: $229.00
Catalog #: E2-680

His6-UBE2G2

Price: 
$229
Quantity: 
100 µg
Data Sheet: 

UBE2G2 is a Class I E2 enzyme that shares 60% and 100% sequence identity with S.cerevisiae UBC7 and mouse UBC7, respectively.  In yeast, UBC7 is a endoplasmic reticulum (ER)-bound molecule with an active site that faces the cystosol. The UBBE2G2 E2 enzyme and the GP78 E3 ligase are active components of ERAD (endoplasmic reticulum-associated degradation) pathway which is essential for the degradation of misfolded ER proteins. The mechanism of K48-linked poly-ubiquitination by UBE2G2/GP78 appears to involve the transfer of preassembled Ubiquitin chains from UBE2G2 to lysine residues on a substrate. The E2 and E3 enzymes form a large hetero-oligomer which brings multiple UBE2G2 molecules into close proximity which allows for Ubiquitin transfer between neighboring E2 molecules.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 7.2, 50 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
MW: 
21 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions. 

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.