E2-678

Material Data Sheet

His6-UbcH9/UBE2E3

Price: $120.00
Catalog #: E2-678

His6-UbcH9/UBE2E3

Price: 
$120 $200
Quantity: 
50 μg 100 μg
Data Sheet: 
application/pdf icon678_Ube2E3.pdf
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 50 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration varies with lot number.

MW: 
24.9 kDa
Purity: 
> 95% by SDS-PAGE

UBE2E3 is a Class III E2 protein with unique C- and N-terminal extension in addition to the conserved UBC domain. This enzyme shares over 90% identity with UbcH6, mouse UbcM2 and Drosophila UbcD2, but the protein N-termini are divergent. This E2 enzyme is active in conjugation reactions with various E3 ligase enzyme (BRCA1, CHIP), and is known to interact with two RING-finger proteins ARA54 and RNF8 via its UBC domain. This protein has an N-terminal His6-tag. (Accession # NP_006348.1)

Use & Storage

Use: 
Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions. 
Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Debonneville C and Staub O. (2004) Mol.Cell Biol. 24:2397-2409
Fotia A.B., et al. (2006) Int J Biochem Cell Biol. 38:472-479
Ito K et al. (1999) Cytogenet. Cell Genet. 84:99-104
Ito K et al. (2001) Eur J Biochem. 268:2725-2732
Matuschewski K., et. al. (1996) J. Biol. Chem. 271:472-479
Plafker K.S., et al. (2004) J. Cell Biol. 167:649-659
Plafker K.S., et al (2008) Invest Ophthalmol Vis Sci. 49:5611-5618