E2-678

Material Data Sheet

His6-UbcH9/UBE2E3

Price: $229.00
Catalog #: E2-678

His6-UbcH9/UBE2E3

Price: 
$229
Quantity: 
100 µg
Data Sheet: 

UBE2E3 is a Class III E2 protein with unique C- and N-terminal extension in addition to the conserved UBC domain. This enzyme shares over 90% identity with UbcH6, mouse UbcM2 and Drosophila UbcD2, but the protein N-termini is divergent. This E2 enzyme is active in conjugation reactions with various E3 ligase enzymes (BRCA1, CHIP) and is known to interact with two RING-finger proteins ARA54 and RNF8 via its UBC domain.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 50 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
MW: 
25 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions. 

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.