E2-677

Material Data Sheet

His6-USE1/UBE2Z

Price: $229.00
Catalog #: E2-677

His6-USE1/UBE2Z

Price: 
$229
Quantity: 
100 µg

USE1 is a recently identified E2 conjugating enzyme which is specifically charged with Ubiquitin by the E1 activating enzyme UBA6. UBA6 has a distinct preference and specificity for USE1 based on the regions specific to the C-terminal Ubiquitin-fold domain. USE1 is not active with the canonical ubiquitin E1 UBA1.  After activating Ubiquitin, UBA6 transfers Ubiquitin to USE1 leading to the formation of a thioester linkage with its catalytic site cysteine (Cys 188). The Ubiquitin can then be conjugated to substrate proteins via specific E3 ligase enzymes.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 50 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
MW: 
43 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions. 

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.