E2-675

Material Data Sheet

His6-UFC1

Price: $229.00
Catalog #: E2-675

His6-UFC1

Price: 
$229
Quantity: 
100 µg
Data Sheet: 

UFC1 has been identified as the E2 conjugating enzyme for the Ubiquitin-like modifier, Ufm1. The protein was originally discovered in HEK293 cells by immunoprecipitation using FLAG-UBA5, the E1 activating enzyme of Ufm1. UFC1 shares no homology to other E2 enzymes except for the region around the active site cysteine (Cys 116) which is capable of forming a thioester bond with Ufm1.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 50 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
MW: 
23 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions. 

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.