E2-675

Material Data Sheet

His6-Ufc1

Price: $120.00
Catalog #: E2-675

His6-Ufc1

Price: 
$120 $200
Quantity: 
50 μg 100 μg
Data Sheet: 
application/pdf icon675_Ufc1.pdf
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 7.5, 50 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration varies with lot number.

MW: 
22.6 kDa
Purity: 
> 95% by SDS-PAGE

Ufc1 has been identified as the E2-like conjugating enzyme for the ubiquitin-like modifier, Ufm1 (UL-500). The protein was originally discovered in HEK293 cells by immunoprecipitation using FLAG-Uba5 (E-319), the E1-like activating enzyme of Ufm1. Ufc1 shares no homology to other E2 enzymes except for the region around the active site cysteine (Cys116) which is capable of
forming a thioester bond with Ufm1. This protein contains an N-terminal His6 tag.

Use & Storage

Use: 
Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions. 
Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Dou, T. et al. (2005) Mol. Biol. Rep. 32: 265-271.
Komatsu, M et al. (2004) EMBO J. 23: 1977-1986.