E2-670

Material Data Sheet

His6-Apg3p/ATG3/Apg3L

Price: $120.00
Catalog #: E2-670

His6-Apg3p/ATG3/Apg3L

Price: 
$120 $200
Quantity: 
50 μg 100 μg
Data Sheet: 
application/pdf icon670_Apg3.pdf
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol. Actual concentration will vary with specific lot number.

MW: 
36.8 kDa
Purity: 
> 95% by SDS-PAGE

Also known as: His6-Apg3p (Apg3L)

Apg3p is the E2-like enzyme necessary for the covalent conjugation of human Apg8 modifier proteins to lipid phosphatidylethanolamine (PE) and is essential in autophagosomal membrane formation. The Apg8 ubiquitin-like proteins (including GABARAP, GATE-16, MAP-LC3) are first activated by the E1-like activating enzyme Apg7p. Apg7p forms a complex with Apg3p which subsequently forms a glycyl thiolester bond with Apg8 proteins through its active site cysteine (C264). Accession # NP_071933.

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Abeliovich H. and Klionsky D.J. (2001) Euk. Cell. 65: 463-479
Ichimura Y., et al. (2000) Nature. 408: 488-492
Reggiori F. and Klionsky D.J. (2002) Euk. Cell. 1: 11-21
Tanida I., et al. (2002) J. Biol. Chem. 277: 13739-13744
Tanida I., et al (2003) Biochem. Biophys. Res. Comm. 300: 637-644