E2-666

Material Data Sheet

His6 UBE2N/UBE2V2 Complex

Price: $229.00
Catalog #: E2-666

His6 UBE2N/UBE2V2 Complex

Price: 
$229
Quantity: 
100 µg
Data Sheet: 

Ubiquitin-­conjugating Enzyme E2N (UBE2N), also known as Ubiquitin­-conjugating Enzyme 13 (Ubc13), forms a functional complex with the catalytically inactive UBE2V2 (human homologue of yeast MMS2) protein.  Human UBE2N/Ubc13 shares 100% and 99% amino acid (aa) sequence identity with the mouse and rat orthologs, respectively, while human UBE2V2 shares 99% aa sequence identity with its mouse and rat orthologs.  The UBE2N/UBE2V2 Complex functions with Ubiquitin ligases (E3s), including RNF111 and RNF8, to synthesize Lys63­linked Ubiquitin chains that can either be unanchored or attached to target proteins.  The UBE2N/UBE2V2 complex has important roles in facilitating responses to various forms of DNA damage.

Product Information

Stock: 
X mg/ml (X µM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 2 mM TCEP
MW: 
18 kDa (UBE2N), 17 kDa (UBE2V2)
Purity: 
>95 % by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain.

Use & Storage

Use: 

Recombinant Human His6­UBE2N/UBE2V2 Complex is a member of the Ubiquitin­conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin­activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins.  Reaction conditions will need to be optimized for each specific application.

Storage: 

Store at -80°C.  Avoid multiple freeze/thaw cycles.