E2-664

Material Data Sheet

His6-UBE2N (Ubc13)/Uev1a complex 

Price: $229.00
Catalog #: E2-664

His6-UBE2N (Ubc13)/Uev1a complex 

Price: 
$229
Quantity: 
100 µg
Data Sheet: 
UBE2N (Ubc13) forms a heterodimeric complex with Uev1, and is able to assemble K63-linked poly-ubiquitin chains which have novel regulatory roles. These ubiquitin chains have a role in a variety of processes such as DNA repair, endocytosis, polysome stability and signal transduction pathways.

Product Information

Stock: 
50 mM Hepes 8, 1 mM DTT, 200mM NaCl, 10% glycerol. Actual concentration will vary with specific lot number.
MW: 
42 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions. 

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Hofmann R.M., et al. (1999) Cell 96:645-653

Hofmann R.M. and Pickart C.M. (2001) J. Biol. Chem. 276:27936-27943

McKenna S.,et al. (2001) J. Biol. Chem. 276:40120-40126

McKenna S., et al. (2003) Biochem. 42:7922-7930

Moraes T.F., et al. (2001) Nat. Struc. Biol. 8:669-673

VanDenmark A.P., et al. (2001) Cell. 105:711-720

Yamaguchi T., et al. (1999) J. Biochem. 120:494-497