E2-662

Material Data Sheet

His6-Uev1a (Mms2)/UBE2V1

Price: $200.00
Catalog #: E2-662

His6-Uev1a (Mms2)/UBE2V1

Price: 
$200
Quantity: 
100 μg
Data Sheet: 
application/pdf icon662_UEV.pdf
Stock: 

X mg/ml (X μM) in PBS, 10% glycerol, 1 mM DTT, pH 7.2. Actual concentration will vary with specific lot number.

MW: 
25 kDa
Purity: 
> 95% by SDS-PAGE
Ubiquitin E2 variant (UEV) proteins are similar to E2s in sequence but lack the active site cysteine residue. Uev1 is the human homolog of the yeast Mms2 protein. This particular UEV protein functions with the canonical E2 UbcH13 in the assembly of K63-linked poly-ubiquitin chains. These ubiquitin chains have a role in a variety of processes such as DNA repair, endocytosis, polysome stability and signal transduction pathways.

Use & Storage

Use: 
Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions. 
Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Broomfield S., et al. (1998) 95:5678-5683

Hofmann R.M., et al. (1999) Cell 96:645-653

Hofmann R.M. and Pickart C.M. (2001) J. Biol. Chem. 276:27936-27943

McKenna S.,et al. (2001) J. Biol. Chem. 276:40120-40126

McKenna S., et al. (2003) Biochem. 42:7922-7930

Moraes T.F., et al. (2001) Nat. Struc. Biol. 8:669-673

VanDenmark A.P., et al. (2001) Cell. 105:711-720

Xiao W., et al. (1998) Nuc. Acids. Res. 26:3908-3914

Yamaguchi T., et al. (1999) J. Biochem. 120:494-497