E2-662

Material Data Sheet

His6-Uev1a/UBE2V1

Price: $229.00
Catalog #: E2-662

His6-Uev1a/UBE2V1

Price: 
$229
Quantity: 
100 µg
Ubiquitin¬conjugating Enzyme Variant 1a (Uev1a), also known as Ubiquitin¬conjugating Enzyme E2 Variant 1 (UBE2V1), is a member of the Ubiquitin¬conjugating (E2) enzyme family. Human UBE2V1 and its mouse ortholog have predicted molecular weights of 19 kDa and 16.5 kDa, respectively. The human protein shares 89% amino acid (aa) sequence identity with its mouse ortholog. The E2 catalytic core domain of UBE2V1 lacks an active site cysteine residue, rendering it catalytically inactive on its own. However, in the cytoplasm UBE2V1 is able to form a catalytically active complex with UBE2N (Ubc13), which mediates the synthesis of K63¬linked Ubiquitin chains and is required for NF¬κB activation. UBE2V1 is required for UBE2N (Ubc13) ¬dependent K63¬linked Ubiquitin chain formation. The human protein contains a non¬conserved 30 aa N¬terminal tail that functionally differentiates it from Mms2, another E2 enzyme variant and UBE2N binding partner. Pharmacological inhibition of the UBE2N/UBE2V1 Complex blocks the proliferation of diffuse large B¬cell lymphoma cells, suggesting that this complex may play a role in cancer. This protein has an N¬terminal His6¬tag.

Product Information

Stock: 
X mg/ml (X μM) in PBS, 10% glycerol, 1 mM DTT, pH 7.2. Actual concentration will vary with specific lot number.
MW: 
25 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions. 

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Broomfield S., et al. (1998) 95:5678-5683

Hofmann R.M., et al. (1999) Cell 96:645-653

Hofmann R.M. and Pickart C.M. (2001) J. Biol. Chem. 276:27936-27943

McKenna S.,et al. (2001) J. Biol. Chem. 276:40120-40126

McKenna S., et al. (2003) Biochem. 42:7922-7930

Moraes T.F., et al. (2001) Nat. Struc. Biol. 8:669-673

VanDenmark A.P., et al. (2001) Cell. 105:711-720

Xiao W., et al. (1998) Nuc. Acids. Res. 26:3908-3914

Yamaguchi T., et al. (1999) J. Biochem. 120:494-497