E2-660

Material Data Sheet

His6-UbcH13/UBE2N

Price: $200.00
Catalog #: E2-660

His6-UbcH13/UBE2N

Price: 
$200
Quantity: 
100 μg
Data Sheet: 
application/pdf icon660_UBE2N Ubc13.pdf
Stock: 

X mg/ml (X μM)in 50 mM HEPES pH 8.0, 150 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration will vary with specific lot number.

MW: 
17 kDa
Purity: 
> 95% by SDS-PAGE

UBE2N (Ubc13) forms a heterodimeric complex with Uev1, and is able to assemble K63-linked
poly-ubiquitin chains which have novel regulatory roles. These ubiquitin chains have a role in a
variety of processes such as DNA repair, endocytosis, polysome stability and signal transduction
pathways.

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions. 

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Hofmann R.M., et al. (1999) Cell 96:645-653
Hofmann R.M. and Pickart C.M. (2001) J. Biol. Chem. 276:27936-27943
McKenna S.,et al. (2001) J. Biol. Chem. 276:40120-40126
McKenna S., et al. (2003) Biochem. 42:7922-7930
Moraes T.F., et al. (2001) Nat. Struc. Biol. 8:669-673
VanDenmark A.P., et al. (2001) Cell. 105:711-720
Yamaguchi T., et al. (1999) J. Biochem. 120:494-497