E2-656

Material Data Sheet

Ubc12/UBE2M

Price: $229.00
Catalog #: E2-656

Ubc12/UBE2M

Price: 
$229
Quantity: 
100 µg
Data Sheet: 

NEDDylation requires the coordinated activities of UBE2M (Ubc12) and the APP-BPI/UBA3 activating enzyme. This E2 enzyme mediates the covalent conjugation of the ubiquitin-like protein NEDD8 to a limited number of cellular proteins such as Cdc53/Cul1 and other members of the cullin family (which are structural subunits of the Skp1-based and elongin B/C-based families of ubiquitin protein ligases). The NEDD8 pathway is essential for cell viability in fission yeast, and in mammalian cells it is essential for cell cycle progression and morphogenesis.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 125 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
MW: 
20 kDa
Purity: 
> 90% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.