E2-656

Material Data Sheet

UbcH12/UBE2M

Price: $120.00
Catalog #: E2-656

UbcH12/UBE2M

Price: 
$120 $200
Quantity: 
50 μg 100 μg
Data Sheet: 
application/pdf icon656_UBE2M Ubc12.pdf
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 125 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration will vary with specific lot number.

MW: 
20 kDa
Purity: 
> 95% by SDS-PAGE

 

NEDDylation requires the coordinated activities of UBE2M (Ubc12) and the APP-BPI/Uba3

activating enzyme. This E2 enzyme mediates the covalent conjugation of the ubiquitin-like

protein NEDD8 to a limited number of cellular proteins such as Cdc53/Cul1 and other members

of the cullin family (which are structural subunits of the Skp1-based and elongin B/C-based

families of ubiquitin protein ligases). The NEDD8 pathway is essential for cell viability in fission

yeast, and in mammalian cells it is essential for cell cycle progression and morphogenesis.

Use & Storage

Use: 
Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.
Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Amir R.E., et al. (2002) J. Biol. Chem. 277: 23253-23259
Gong L., et al. (1999) J. Biol. Chem. 274: 12036-12042
Hochstrasser M. (2000) Nat. Cell. Biol. 2: E153-E157
Osaka F., et al (1998) Genes. Dev. 12:2263-2268
Podust V.N., et al. (2000) Proc. Natl. Acad. Sci. 97:4579-4584
Tateishi K., et al. (2001) J. Cell. Biol. 155:571-580
Wada H., et al. (2000) J. Biol. Chem. 275: 17008-17015