Material Data Sheet

Ubc12/UBE2M, GST tag

Price: $239.00
Catalog #: E2-655

Ubc12/UBE2M, GST tag

100 μg
NEDDylation requires the coordinated activities of UBE2M (Ubc12) and the APP-BPI/Uba3 activating enzyme. This E2 enzyme mediates the covalent conjugation of the ubiquitin-like protein NEDD8 to a limited number of cellular proteins such as Cdc53/Cul1 and other members of the cullin family (which are structural subunits of the Skp1-based and elongin B/C-based families of ubiquitin protein ligases). The NEDD8 pathway is essential for cell viability in fission yeast, and in mammalian cells it is essential for cell cycle progression and morphogenesis.

Product Information

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 125 mM NaCl, 10% glycerol, 1 mM DTT
46 kDa
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage


Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human GST-UBE2M/Ubc12 concentration of 0.1-1 μM.


Store at -80°C. Avoid multiple freeze/thaw cycles.