Material Data Sheet


Price: $0.00
Catalog #: E2-654


0.00 239.00
100 µg
Data Sheet: 

Ubiquitin­conjugating Enzyme H10 (UbcH10), also known as Ubiquitin­-conjugating Enzyme E2C (UBE2C), is a 179 amino acid (aa) member of the yeast Ubc4/5 family of Ubiquitin­Conjugating  Enzymes (E2) and has a predicted molecular weight of 20 kDa.  Human UBE2C shares 96% aa sequence identity with mouse and rat UBE2C.  UBE2C is an essential mediator of mitotic destruction events and cell cycle progression.  UBE2C recognizes TEK sequences in target proteins such as Cyclins A and B, mediates Lys11-­linked ubiquitination, and promotes target protein degradation in conjunction with APC/C, a Ubiquitin Ligase (E3).  The catalytic activity of UBE2C is regulated by a conserved N­-terminal extension, which mediates E2-­E3 interaction.  UBE2C is overexpressed in a variety of human cancers, and alternate splice isoforms may contribute to uncontrolled cell proliferation and tumor progression.  

Product Information

0.50 mg/ml (25 µM) in 50 mM HEPES pH 7.0, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
20 kDa
> 95% by SDS-PAGE under reducing conditions and visualized with Colloidal Coomassie Blue stain

Use & Storage


Recombinant Human UbcH10/UBE2C is a member of the Ubiquitin­conjugating Enzyme (E2) family that receives Ubiquitin from a Ubiquitin­Activating Enzyme (E1) and subsequently interacts with a Ubiquitin ligase (E3) to conjugate ubiquitin to substrate proteins.  Reaction conditions will need to be optimized for each specific application.


Store at -80°C.  Avoid multiple freeze-thaw cycles.