E2-654

Material Data Sheet

UbcH10/UBE2C

Price: $229.00
Catalog #: E2-654

UbcH10/UBE2C

Price: 
$229
Quantity: 
100 µg
Data Sheet: 

Ubiquitin­conjugating Enzyme H10 (UbcH10), also known as Ubiquitin­conjugating Enzyme E2C (UBE2C), is a 179 amino acid (aa) member of the yeast Ubc4/5 family of Ubiquitin­Conjugating  Enzymes (E2) and has a predicted molecular weight of 20 kDa.  Human UbcH10/UBE2C shares 96% aa sequence identity with mouse and rat UBE2C.  UbcH10/UBE2C is an essential mediator of mitotic destruction events and cell cycle progression.  UbcH10/UBE2C recognizes TEK sequences in target proteins such as Cyclins A and B, mediates Lys11­linked ubiquitination, and promotes target protein degradation in conjunction with APC/C, a Ubiquitin Ligase (E3).  The catalytic activity of UbcH10/UBE2C is regulated by a conserved N­terminal extension, which mediates E2­E3 interaction.  UbcH10/UBE2C is overexpressed in a variety of human cancers, and alternate splice isoforms may contribute to uncontrolled cell proliferation and tumor progression.  

Product Information

Stock: 
0.50 mg/ml (25 mM) in 50 mM HEPES, pH 7.0, 200 mM NaCl, 1 mM TCEP, and 10% glycerol
MW: 
20 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized with Colloidal Coomassie Blue stain

Use & Storage

Use: 

Recombinant Human UbcH10/UBE2C is a member of the Ubiquitin­conjugating Enzyme (E2) family that receives Ubiquitin from a Ubiquitin­Activating Enzyme (E1) and subsequently interacts with a Ubiquitin ligase (E3) to conjugate ubiquitin to substrate proteins.  Reaction conditions will need to be optimized for each specific application.

Storage: 

Store at -80°C.  Avoid multiple freeze-thaw cycles.