E2-650

Material Data Sheet

His6-UbcH10/UBE2C

Price: $120.00
Catalog #: E2-650

His6-UbcH10/UBE2C

Price: 
$120 $200
Quantity: 
50 μg 100 μg
Data Sheet: 
application/pdf icon650_H10.pdf
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 125 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration will vary with specific lot number.

MW: 
20 kDa
Purity: 
> 95% by SDS-PAGE

UbcH10 is an essential mediator of mitotic destruction events and cell cycle progression. It catalyzes the destruction of cyclins A and B in conjunction with the anaphase-promoting complex. This activity is essential at the end of mitosis for the inactivation of their partner kinase Cdc2 and exit from mitosis into G1 of the next cell cycle.

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions. 

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Bastians H., et al. (1999) Mol. Biol. Cell. 10:3927-3941
Hershko A. and Ciechanover A. (1998) Ann. Rev. Biochem. 67:425-479
Lin Y., et al. (2002) J. Biol. Chem. 277:21913-21921
Okamoto Y., et al. (2003) Canc. Res. 63:4167-4173
Townsley F.M., et al. (1997) Proc. Natl. Acad. Sci. 94:2362-2367