E2-650

Material Data Sheet

His6-UbcH10/UBE2C

Price: $229.00
Catalog #: E2-650

His6-UbcH10/UBE2C

Price: 
$229
Quantity: 
100 µg

Ubiquitin­conjugating Enzyme H10 (UbcH10), also known as Ubiquitin­conjugating Enzyme E2C (UBE2C), is a 179 amino acid (aa) member of the yeast Ubc4/5 family of Ubiquitin­Conjugating  Enzymes (E2) and has a predicted molecular weight of 20 kDa.  Human UbcH10/UBE2C shares 96% aa sequence identity with mouse and rat UBE2C.  UbcH10/UBE2C is an essential mediator of mitotic destruction events and cell cycle progression.  UbcH10/UBE2C recognizes TEK sequences in target proteins such as Cyclins A and B, mediates Lys11­linked ubiquitination, and promotes target protein degradation in conjunction with APC/C, a Ubiquitin Ligase (E3).  The catalytic activity of UbcH10/UBE2C is regulated by a conserved N­terminal extension, which mediates E2­E3 interaction.  UbcH10/UBE2C is overexpressed in a variety of human cancers, and alternate splice isoforms may contribute to uncontrolled cell proliferation and tumor progression.  Note:  in some applications, a His6-tag has been reported to interfere with UbcH10 function.  This protein has been tested for Ubiquitin charging only.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 125 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
MW: 
20 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions. 

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.