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Material Data Sheet
UBE2I/Ubc9
Price: $120.00
UBE2I/Ubc9
Price:
$120 $200
Quantity:
50 μg 100 μg
Data Sheet:
UBE2I (Ubc9) is a member of the E2 family and is homologous to ubiquitin-conjugating enzymes, but is specific for the conjugation of SUMO to a variety of target proteins. This E2 is unusual in that it interacts directly with protein substrates that are modified by SUMOylation, and may play a role in substrate recognition. Ubc9 can mediate the conjugation of SUMO1, SUMO2 and SUMO3 to a variety of proteins including RanGAP1, IB, and PML without the requirement of an E3 ligase.
Product Information
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol.
MW:
20 kDa
Purity:
> 95% by SDS-PAGE Use & Storage
Use:
Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.
Literature
References:
Desterro J.M., et al. (1999) J. Biol. Chem. 274:10618-24
Joana M.P, et al. (1997) FEBS. Lett. 417:3168-3179
Johnson E.S and Blobel G. (1997) J. Biol. Chem. 272:26799-26794
Ito K., et al. (1999) Cytogenet. Cell. 84:99-104
Liu Q., et al. (1999) J. Biol. Chem. 274:16979-16987
Matushewski K., et al. (1996) J. Biol. Chem. 271:2789-2794
Sampson D.A., et al. (2001) J. Biol. Chem. 276:21664-21669
Tatham M.H., et al. (2003) Biochem. 42:3168-3179
Tong H., et al. (1997) J. Biol. Chem. 272:21381-21387
Watanabe T.K., et al. (1996) Cytogen. Cell. Genet. 72:86-89
Yasugi T and Howley P.M (1996) Nuc. Acid. Res. 24:2005-2010
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