E2-645

Material Data Sheet

UbcH9/UBE2E3

Price: $120.00
Catalog #: E2-645

UbcH9/UBE2E3

Price: 
$120 $200
Quantity: 
50 μg 100 μg
Data Sheet: 
application/pdf icon645_UBE2I Ubc9.pdf
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 7.6, 125 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration will vary with specific Lot #.

MW: 
20 kDa
Purity: 
> 95% by SDS-PAGE
UBE2I (Ubc9) is a member of the E2 family and is homologous to ubiquitin-conjugating enzymes, but is specific for the conjugation of SUMO to a variety of target proteins. This E2 is unusual in that it interacts directly with protein substrates that are modified by SUMOylation, and may play a role in substrate recognition. UbcH9 can mediate the conjugation of SUMO-1, SUMO-2 and SUMO-3 to a variety of proteins including RanGAP1, IkBa, and PML without the requirement of an E3 ligase.

Use & Storage

Use: 
Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.
Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Desterro J.M., et al. (1999) J. Biol. Chem. 274:10618-24
Joana M.P, et al. (1997) FEBS. Lett. 417:3168-3179
Johnson E.S and Blobel G. (1997) J. Biol. Chem. 272:26799-26794
Ito K., et al. (1999) Cytogenet. Cell. 84:99-104
Liu Q., et al. (1999) J. Biol. Chem. 274:16979-16987
Matushewski K., et al. (1996) J. Biol. Chem. 271:2789-2794
Sampson D.A., et al. (2001) J. Biol. Chem. 276:21664-21669
Tatham M.H., et al. (2003) Biochem. 42:3168-3179