E2-645

Material Data Sheet

UBE2I (Ubc9)

Price: $229.00
Catalog #: E2-645

UBE2I (Ubc9)

Price: 
$229
Quantity: 
100 µg
Data Sheet: 
UBE2I (Ubc9) is a member of the E2 family and is homologous to ubiquitin-conjugating enzymes, but is specific for the conjugation of SUMO to a variety of target proteins. This E2 is unusual in that it interacts directly with protein substrates that are modified by SUMOylation, and may play a role in substrate recognition. In some cases, Ubc9 can mediate the conjugation of SUMO1, SUMO2 and SUMO3 to a variety of proteins including RanGAP1, IkBa, and PML without the requirement of an E3 ligase, although for some substrates a SUMO E3 ligase is required.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 7.6, 125 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
MW: 
20 kDa
Purity: 
> 90% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.