Material Data Sheet
Quantity:50 μg 100 μg
UbcH8 can be charged with both ubiquitin and ISG15 via the activities of the respective E1Ub and E1ISG15 enzymes. UbcH8 is highly homologous to UbcH7, and functions in ubiquitin conjugation reactions and in HECT E3 (such as E6AP) and RING-FINGER (such as Parkin) protein mediated events. UbcH8 is the major E2 for ISG15 conjugation in reactions initiated by the ISG15-specific E1 activating enzyme. ISG15 is an ubiquitin-like protein that is conjugated to cellular proteins after IFN-α/β stimulation.
Stock:X mg/ml (XμM) in 50 mM HEPES pH 8.0, 100 mM NaCl,10% glycerol. Actual concentration will vary with specific Lot #.
Purity:> 95% by SDS-PAGE
Use & Storage
Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.
Store at -80°C. Avoid multiple freeze/thaw cycles.
Ardley H.C., et al. (2000) Cytogenet. Cell. Genet. 89:137-140
Kumar S., et al.. (1997) J. Biol. Chem. 272:13548-13554
Nyman T.A., et al.. (2000) Eur. J. Biochem. 267:4011-4019
Zhao C., et al.. (2004) Proc. Natl. Acad. Sci. 101:7578-7582
Zhang Y., et al.. (2000) Proc. Natl. Acad. Sci. 97:13354-13359