E2-643

Material Data Sheet

GST-UbcH8/UBE2L6

Price: $120.00

Catalog #: E2-643

GST-UbcH8/UBE2L6

Price:

$120 $150

Quantity:

50 μg 100 μg

Data Sheet:

643_50ug.pdf

Stock:

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol. Actual concentration will vary with specific Lot #

MW:

44 kDa

Purity:

> 95% by SDS-PAGE

UbcH8 can be charged with both ubiquitin and ISG15 via the activities of the respective E1^Ub and E1^ISG15 enzymes. UbcH8 is highly homologous to UbcH7, and functions in ubiquitin conjugation reactions and in HECT E3 (such as E6AP) and RING-FINGER (such as Parkin) protein mediated events. UbcH8 is the major E2 for ISG15 conjugation in reactions initiated by the ISG15-specific E1 activating enzyme. ISG15 is an ubiquitin-like protein that is conjugated to cellular proteins after IFN-α/β stimulation.

Use & Storage

Use:

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.

Storage:

Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References:

Ardley H.C., et al. (2000) Cytogenet. Cell. Genet. 89:137-140
Kumar S., et al.. (1997) J. Biol. Chem. 272:13548-13554
Nyman T.A., et al.. (2000) Eur. J. Biochem. 267:4011-4019
Zhao C., et al.. (2004) Proc. Natl. Acad. Sci. 101:7578-7582
Zhang Y., et al.. (2000) Proc. Natl. Acad. Sci. 97:13354-13359