E2-632

Material Data Sheet

His6-UbcH6/UBE2E1 Dominant Negative

Price: $180.00
Catalog #: E2-632

His6-UbcH6/UBE2E1 Dominant Negative

Price: 
$180
Quantity: 
100 μg
Data Sheet: 
application/pdf icon632_dnH6.pdf
Stock: 

X mg/ml (X μM) in PBS, 10% glycerol, 1 mM DTT, pH 7.2. Actual concentration will vary with specific lot number.

MW: 
21 kDa
Purity: 
> 95% by SDS-PAGE

UbcH6 mediates the selective degradation of short-lived and abnormal proteins. The enzyme has high similarity to UbcH5 E2 subfamily, but it does not participate in HECT-domain mediated events. This enzyme has a mutation of the active site from cysteine to serine which abolishes the ability of UbcH6 to transfer ubiquitin to an accepting E3 protein. Ideal for use as a negative or competitive control, or to study protein-protein interactions. This protein has an N-terminal His6 tag.

Use & Storage

Use: 
Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.
Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Nuber U. And Scheffner M. (1999) J. Biol. Chem. 274:7576-7582