E2-630

Material Data Sheet

His6-UbcH6/UBE2E1

Price: $229.00
Catalog #: E2-630

His6-UbcH6/UBE2E1

Price: 
$229
Quantity: 
100 µg
Data Sheet: 

Ubiquitin-conjugating Enzyme H6 (UbcH6), also known as Ubiquitin-conjugating Enzyme E2E-1 (UBE2E1), is a ubiquitously expressed protein with a predicted molecular weight of 21 kDa. Human UBE2E1 shares 99% and 100% amino acid sequence identity with mouse and rat UBE2E1, respectively. UBE2E1 mediates the selective degradation of short-lived and abnormal proteins.  UBE2E1 catalytic activity is modulated following the addition of the Ubiquitin-like protein ISG15 to Lys136. In the nucleus, the N-terminal fragment of UBE2E1 interacts with the Ubiquitin ligase (E3) TRIM21 to mediate substrate ubiquitination.  UBE2E1 also interacts with the viral E3, ICP0, during HSV-1 infection.  In addition, UBE2E1-dependent protein ubiquitination is implicated in polyglutamine tract disorders such as ataxia.

Product Information

Stock: 
X mg/ml (X µM) in 50 mM HEPES pH 8.0, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
MW: 
24 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.