E2-625

Material Data Sheet

GST-UbcH5c/UBE2D3

Price: $120.00
Catalog #: E2-625

GST-UbcH5c/UBE2D3

Price: 
$120 $200
Quantity: 
50 μg 100 μg
Data Sheet: 
application/pdf icon625_gstH5c.pdf
Stock: 

[E2] = X mg/ml (X μM) in 50 mM HEPES pH 7.6, 50 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration varies with lot number.

MW: 
43 kDa
Purity: 
> 95% by SDS-PAGE
UbcH5 enzymes are human homologs of the yeast UBC4/5 family and play many important regulatory roles in inflammation and cancer. UbcH5a mediates the degradation of a myriad of short-lived regulatory proteins (such as p53 in the presence of E6/E6-AP or MDM2, c-Fos, IκBα, p105) and abnormal proteins. UbcH5c has 88% and 89% sequence identity with UbcH5a and UbcH5b respectively. This protein has an N-terminal GST tag.

Use & Storage

Use: 
Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.
Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Brzovic P.S., et al. (2006) Cell Cycle 5:2867-2873

Jensen J., et al. (1995) J. Biol. Chem. 270:30408-30414

Gehrke S.G., et al. (2003) Blood. 101:3288-3293

Nuber U. And Scheffner M. (1999) J. Biol. Chem. 274:7576-7582

Scheffner M., et al. (1994) Proc. Natl. Acad. Sci. 91:8797-8801

Schwarz S.E., et al. (1998) J. Biol. Chem. 273:12148-12154