E2-615

Material Data Sheet

GST-UbcH5a/UBE2D1

Price: $229.00
Catalog #: E2-615

GST-UbcH5a/UBE2D1

Price: 
$229
Quantity: 
100 µg
Data Sheet: 

Ubiquitin-conjugating Enzyme H5a (UbcH5a), also known as Ubiquitin-conjugating Enzyme E2D 1 (UBE2D1), is a ubiquitously expressed protein that is related to Stimulator of Iron Transport (SFT). Human UBE2D1 has a predicted molecular weight of 17 kDa and shares 89% and 88% amino acid (aa) sequence identity with the related family members UbcH5b and UbcH5c, respectively.  Human UBE2D1 shares 100% aa sequence identity with the mouse and rat orthologs.  UBE2D1 has a conserved E2 catalytic core domain that contains an active site cysteine residue, and it interacts with a variety of HECT and RING finger Ubiquitin ligases (E3) to mediate the ubiquitination of specific target proteins. UbcH5a/UBE2D1 interacts with the E3, E6AP, to conjugate Ubiquitin to the tumor suppressor, p53. Additional protein targets of UbcH5a/UBE2D1 include cFos, RIP1, and HIF1. Pathologically, UBE2D1 is implicated in protein degradation during cancer and immune responses.  This protein contains an amino-terminal GST tag.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
MW: 
43 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Reaction conditions will need to be optimized for each specific application. We recommend an initial UBE2D1 concentration of 0.1-1 μM.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.