E2-613

Material Data Sheet

His6-HR6B/UBE2B

Price: $229.00
Catalog #: E2-613

His6-HR6B/UBE2B

Price: 
$229
Quantity: 
100 µg

Also known as: His6-hHR6B (human Homolog of RAD6B)

The human HR6A and HR6B proteins share about 95% amino acid sequence identity with each other and about 70% with yeast counterparts, but notably lack the acidic C-terminal domain found in S. cerevisiae RAD6. The RAD6 pathway is essential to post-replication repair of DNA and damage-induced mutagenesis in eukaryotic cells. hHR6 protein expression is cell cycle regulated and function with RAD5 and RAD18 RING-Finger proteins. The human proteins function similar to the yeast homologs, and may have additional roles in chromatin remodeling and spermatogenesis. This protein has an N-terminal His6 tag.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 7.8, 50 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
MW: 
20 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.