E2-612

Material Data Sheet

His6-HR6A/UBE2A

Price: $229.00
Catalog #: E2-612

His6-HR6A/UBE2A

Price: 
$229
Quantity: 
100 µg

Also known as: His6-hHR6A (human Homolog of RAD6A)

The human HR6A and HR6B proteins share about 95% amino acid sequence identity with each other and about 70% with yeast counterparts, but notably lack the acidic C-terminal domain found in S. cerevisiae proteins. The RAD6 pathway is essential to post-replication repair of DNA and damage-induced mutagenesis in eukaryotic cells. hHR6 protein expression is cell cycle regulated and function with RAD5 and RAD18 RING-finger proteins. The human proteins function similar to the yeast homologs, and may have additional roles in chromatin remodeling and spermatogenesis Residue Ser120 is an important regulatory site in hHR6A, being phosphorylated in vitro by CDK1/2 on which increases ubiquitin conjugating activity. A nonsense mutation in hHR6A has been linked to a novel X-linked mental retardation syndrome (XMLR). This protein has an N-terminal His6 tag.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 7.8, 50 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
MW: 
20 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.