E2-611

Material Data Sheet

His6-UbcH3/Cdc34 Dominant Negative

Price: $180.00
Catalog #: E2-611

His6-UbcH3/Cdc34 Dominant Negative

Price: 
$180
Quantity: 
100 μg
Data Sheet: 
application/pdf icon611_dnH3.pdf
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 50 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration will vary with specific lot number.

MW: 
27 kDa
Purity: 
> 95% by SDS-PAGE

UbcH3 plays an essential role in the progression of cells from the G1 to S phase of the cell division cycle. One pathway (requiring Cdc34) initiates DNA replication by degrading a CDK (cyclin-dependent kinase) inhibitor. The second pathway, involves the anaphase-promoting complex (APC) initiates chromosome segregation and exit from mitosis by degrading anaphase inhibitors and mitotic cyclins. The active site of this enzyme has been chemically inactivated for use as a negative or competitive control.

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Goebl M.G., et al. (1988) Science 241:1331-1335
Gonen H., et al.. (1999) J. Biol. Chem. 274:14823-14830
King R. W., et al.. (1996) Science 274:1652-1659
Listwan J., et al.. (1998) EMBO. J. 17:368-383
Pintard L., et al.. (2003) Nat. Cell. Biol. 5:856-857
Plon S.E., et al.. (1993) Proc. Natl. Acad. Sci. 90:10484-10488
Ptak C., et al.. (1994) J. Biol. Chem. 269:26539-26545
Seol J.H., et al.. (1999) . Gene. Dev13:1614-1626
Varelas X., et al.. (2003) Mol. Cell. Biol. 23:5388-5400