E2-610

Material Data Sheet

His6-UbcH3/Cdc34/UBE2R1

Price: $229.00
Catalog #: E2-610

His6-UbcH3/Cdc34/UBE2R1

Price: 
$229
Quantity: 
100 µg

UbcH3 plays an essential role in the progression of cells from the G1 to S phase of the cell division cycle. One pathway (requiring Cdc34) initiates DNA replication by degrading a CDK (cyclin-dependent kinase) inhibitor. The second pathway, involves the anaphase-promoting complex (APC) initiates chromosome segregation and exit from mitosis by degrading anaphase inhibitors and mitotic cyclins.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 50 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
MW: 
27 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.